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Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
18/06/2012 |
Data da última atualização: |
18/06/2012 |
Autoria: |
PARKER, M.; RODGERS, J.; LUDTKE, C. B.; KOLESAR, R. |
Título: |
Abate humanitário de suínos. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Rio de Janeiro: WSPA, 2009. |
Descrição Física: |
1 DVD (05,15 min), son, color. |
Idioma: |
Português |
Notas: |
Narração: Roberto G. Engelbrech. Produção WSPA e Animal-I. |
Palavras-Chave: |
Área de descanso; Bem-estar animal; Condição física; Qualidade da carne; Sistema elétrico. |
Thesagro: |
Abate; Manejo; Sangria; Suíno. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00714ngm a2200265 a 4500 001 1926593 005 2012-06-18 008 2009 bl --- 0-- m #d 100 1 $aPARKER, M. 245 $aAbate humanitário de suínos. 260 $aRio de Janeiro: WSPA$c2009 300 $c1 DVD (05,15 min), son, color. 500 $aNarração: Roberto G. Engelbrech. Produção WSPA e Animal-I. 650 $aAbate 650 $aManejo 650 $aSangria 650 $aSuíno 653 $aÁrea de descanso 653 $aBem-estar animal 653 $aCondição física 653 $aQualidade da carne 653 $aSistema elétrico 700 1 $aRODGERS, J. 700 1 $aLUDTKE, C. B. 700 1 $aKOLESAR, R.
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Embrapa Suínos e Aves (CNPSA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
20/09/2011 |
Data da última atualização: |
01/03/2013 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; COOKE, P.; WOOD, D. |
Afiliação: |
CELLI RODRIGUES MUNIZ, CNPAT; FRANCISCO DAS CHAGAS OLIVEIRA FREIRE, CNPAT; FRANCISCO MARTO PINTO VIANA, CNPAT; JOSE EMILSON CARDOSO, CNPAT; PETER COOKE, USDA; DELILAH WOOD, USDA. |
Título: |
Colonization of cashew plants by Lasiodiplodia theobromae: microscopical features. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Micron, v. 42, n. 5, p. 419-428 , 2011. |
Idioma: |
Inglês |
Conteúdo: |
Lasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in Brazil. In an attempt to investigate the ultrastructural features of the pathogen colonization and its response to immunofluorescence labeling, light, confocal and electron microscope studies were conducted on different severity scale patterns of diseased plants. Lasiodiplodia-antisera was checked for cross reactivity against common cashew plants fungi. Optical microscopy analysis revealed a longitudinally sectioned hyphae located within the xylem vessels, showing an extensive hyphal development in the secondary xylem tissue. SEM images demonstrated that the fungus was found in some asymptomatic samples, particularly within the xylem vessels as confirmed by the optical images. Symptomatic sample images showed an extensive distribution of the fungus along the secondary xylem, within the vessels, infecting xylem parenchyma. A closer look in the secondary xylem parenchyma reveals a heavy and profuse invasion of the cells with a distinguishable cell wall disintegration and fully hyphae dispersal. There was no reactivity of Lasiodiplodia-antisera against mycelial extracts of Colletotrichum gloeosporioides, Phomopsis anardii and Pestalotiopsis guepinii. Following incubation of sections with the polyclonal antisera, the hyphae were intensely and regularly labeled. Rays, vessels and parenchyma cells were the preferred pathway for L. theobromae colonization. Artificial infection provides the information that the vascular cylinder is undoubtedly employed and used by the fungus for hyphae distribution. Immunofluorescence assay employed in situ was applied and the polyclonal antisera produced was able to recognize the fungus and proved to be a sensitive technique to detect it. MenosLasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in Brazil. In an attempt to investigate the ultrastructural features of the pathogen colonization and its response to immunofluorescence labeling, light, confocal and electron microscope studies were conducted on different severity scale patterns of diseased plants. Lasiodiplodia-antisera was checked for cross reactivity against common cashew plants fungi. Optical microscopy analysis revealed a longitudinally sectioned hyphae located within the xylem vessels, showing an extensive hyphal development in the secondary xylem tissue. SEM images demonstrated that the fungus was found in some asymptomatic samples, particularly within the xylem vessels as confirmed by the optical images. Symptomatic sample images showed an extensive distribution of the fungus along the secondary xylem, within the vessels, infecting xylem parenchyma. A closer look in the secondary xylem parenchyma reveals a heavy and profuse invasion of the cells with a distinguishable cell wall disintegration and fully hyphae dispersal. There was no reactivity of Lasiodiplodia-antisera against mycelial extracts of Colletotrichum gloeosporioides, Phomopsis anardii and Pestalotiopsis guepinii. Following incubation of sections with the polyclonal antisera, the hyphae were intensely and regularly labeled. Rays, vessels and parenchyma cells were the preferred pathway for L. theobromae colonization. Artificial in... Mostrar Tudo |
Palavras-Chave: |
Anacardium occidentale L; Cell wall degradation; Endophytic fungi; Immunofluorescence. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02449naa a2200229 a 4500 001 1900945 005 2013-03-01 008 2011 bl uuuu u00u1 u #d 100 1 $aMUNIZ, C. R. 245 $aColonization of cashew plants by Lasiodiplodia theobromae$bmicroscopical features. 260 $c2011 520 $aLasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in Brazil. In an attempt to investigate the ultrastructural features of the pathogen colonization and its response to immunofluorescence labeling, light, confocal and electron microscope studies were conducted on different severity scale patterns of diseased plants. Lasiodiplodia-antisera was checked for cross reactivity against common cashew plants fungi. Optical microscopy analysis revealed a longitudinally sectioned hyphae located within the xylem vessels, showing an extensive hyphal development in the secondary xylem tissue. SEM images demonstrated that the fungus was found in some asymptomatic samples, particularly within the xylem vessels as confirmed by the optical images. Symptomatic sample images showed an extensive distribution of the fungus along the secondary xylem, within the vessels, infecting xylem parenchyma. A closer look in the secondary xylem parenchyma reveals a heavy and profuse invasion of the cells with a distinguishable cell wall disintegration and fully hyphae dispersal. There was no reactivity of Lasiodiplodia-antisera against mycelial extracts of Colletotrichum gloeosporioides, Phomopsis anardii and Pestalotiopsis guepinii. Following incubation of sections with the polyclonal antisera, the hyphae were intensely and regularly labeled. Rays, vessels and parenchyma cells were the preferred pathway for L. theobromae colonization. Artificial infection provides the information that the vascular cylinder is undoubtedly employed and used by the fungus for hyphae distribution. Immunofluorescence assay employed in situ was applied and the polyclonal antisera produced was able to recognize the fungus and proved to be a sensitive technique to detect it. 653 $aAnacardium occidentale L 653 $aCell wall degradation 653 $aEndophytic fungi 653 $aImmunofluorescence 700 1 $aFREIRE, F. das C. O. 700 1 $aVIANA, F. M. P. 700 1 $aCARDOSO, J. E. 700 1 $aCOOKE, P. 700 1 $aWOOD, D. 773 $tMicron$gv. 42, n. 5, p. 419-428 , 2011.
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